Seed region sgrna 343915-Seed region sgrna

(A) Binding sites of amiRNAs in different regions of sgRNA Region 1 represents the spacer sequence, and regions 2 and 3 are located in the sgRNA backbone (B) Effects of amiRNAs on the expression of naked sgRNA by qRT PCR GAPDH was used as a control Data are the mean ± SD from five experimentsEncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the nonseed region Depositor Vincent Noireaux Insert sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the nonseed region Use Crispr s Expression Mutation Promoter J Availability Unlike the proximal region, the PAMdistal region has been considered less important in determining the sgRNA specificity However, in our study, we show that this region may actually contribute

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Seed region sgrna

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