(A) Binding sites of amiRNAs in different regions of sgRNA Region 1 represents the spacer sequence, and regions 2 and 3 are located in the sgRNA backbone (B) Effects of amiRNAs on the expression of naked sgRNA by qRT PCR GAPDH was used as a control Data are the mean ± SD from five experimentsEncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the nonseed region Depositor Vincent Noireaux Insert sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the nonseed region Use Crispr s Expression Mutation Promoter J Availability Unlike the proximal region, the PAMdistal region has been considered less important in determining the sgRNA specificity However, in our study, we show that this region may actually contribute
Sequence Recognition And Structure Of Synthetic Crispr Rna A Download Scientific Diagram
